Thin-layer chromatography TLC is a chromatography technique used to separate non-volatile mixtures. This layer of adsorbent is known as the stationary phase. After the sample has been applied on the plate, a solvent or solvent mixture known as the mobile phase is drawn up the plate via capillary action. Because different analytes ascend the TLC plate at different rates, separation is achieved. For example, with silica gel, a very polar substance, non-polar mobile phases such as heptane are used. The mobile phase may be a mixture, allowing chemists to fine-tune the bulk properties of the mobile phase.
Rapid and precise estimation of biodiesel by high performance thin layer chromatography
Thin-layer chromatography - Wikipedia
Preeclampsia is accompanied by an extensive remodeling of the extracellular matrix of umbilical cord. It is associated with an increase in collagen content in the umbilical cord artery. Furthermore, preeclampsia distinctly reduces proteolytic and gelatinolytic activity, especially after activation with various agents. We decided to develop a method for separation and determination of fatty acids from different tissues by high-performance liquid chromatography. That method allowed us to determine cholesteryl ester composition and content in umbilical cord arteries. Studies were performed on the umbilical cord arteries taken from 10 newborns delivered by healthy mothers and 10 newborns delivered by mothers with preeclampsia. Cholesteryl esters were isolated by thin layer chromatography.
The purification of lipids has become simple and convenient. Some twenty years ago HPLC replaced preparative gas chromatography as the method of choice for the analysis of lipids. Additionally thin layer chromatography is frankly and inexpensive analytical tool, a feature having great merit in times of budgetary constraint.
In this paper, a new thin-layer chromatography TLC method for the determination of individual volatile fatty acids VFAs is presented. The experimental procedure is based on the derivatization of VFAs with activating agents and a naphthalene-based amine to form an amide derivative, followed by fast TLC separation of these VFA derivatives and visualization with phosphomolybdic acid at room temperature. Quantification is then performed by densitometric measurements using free software after taking TLC pictures in a home-made black box for homogeneous and reproducible lighting of the plates.